Derivation, Expansion and Characterization of Clinical Grade Mesenchymal Stem Cells from Umbilical Cord Matrix Using Cord Blood Serum
نویسندگان
چکیده
منابع مشابه
Nanofiber Expansion of Umbilical Cord Blood Hematopoietic Stem Cells
Background The aim of this study was the ex vivo expansion of Umbilical Cord Blood hematopoietic stem cells on biocompatible nanofiber scaffolds. Materials and Methods CD133+ hematopoietic stem cells were separated from umbilical cord blood using MidiMacs (positive selection) system by means of monocolonal antibody CD133 (microbeads) subsequently, flowcytometry method was done to asses...
متن کاملnanofiber expansion of umbilical cord blood hematopoietic stem cells
background the aim of this study was the ex vivo expansion of umbilical cord blood hematopoietic stem cells on biocompatible nanofiber scaffolds. materials and methods cd133+ hematopoietic stem cells were separated from umbilical cord blood using midimacs (positive selection) system by means of monocolonal antibody cd133 (microbeads) subsequently, flowcytometry method was done to assess the pur...
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Ex vivo expansion of human umbilical cord blood cells (HUCBC) is explored by several investigators to enhance the repopulating potential of HUCBC. The proliferation and expansion of human hematopoietic stem cells (HSC) in ex vivo culture was examined with the goal of generating a suitable clinical protocol for expanding HSC for patient transplantation. Using primary human mesenchymal stem ...
متن کاملHypoxia Preconditioning Promotes Survival And Clonogenic Capacity Of Human Umbilical Cord Blood Mesenchymal Stem Cells
Background: In recent decade, human umbilical cord blood derived mesenchymal stem cells (hUCB-MSCs) provide enormous potential for appropriate cell therapy, but they have limited growth potential and cease to proliferate due to cellular senescence, so providing a strategy for increasing the stem cell survival is necessary. Methods: In this investigation, MSCs characterized by flow cytome...
متن کاملStudy of telomerase activity, proliferation and differentiation characteristics in umbilical cord blood mesenchymal stem cells
In recent years, considerable advances have been made in the field of regenerative medicine. Unlikeembryonic stem cells, which pose the problems of ethical concerns and cause severe immunological reactions as well as neoplasma formation after transplantation, umbilical cord blood is a primitive source ofmesenchymal stem cells that covers the benefits of both embryonic and adult stem cells. It h...
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ژورنال
عنوان ژورنال: International Journal of Stem Cells
سال: 2010
ISSN: 2005-3606,2005-5447
DOI: 10.15283/ijsc.2010.3.2.119